24 October 2025

RASOPTA Updates 2025

RASOPTA (Recirculated Aquaculture Systems Optimisation for Production and Training in Aquaculture) is an EU-funded Marie Skłodowska-Curie ITN project coordinated by the University of Copenhagen. RASOPTA officially concluded in August 2025, marking the end of its 4-year run

Several PhD students (Early Stage Researchers) have successfully defended their theses. Their work focused on improving water quality, reducing off-flavours, and enhancing fish health in recirculating aquaculture systems (RAS)  systems and developing new tools.

 

 

 

Water Quality: Studies on biofilter microbiomes revealed that cleaning moving bed biofilters can unintentionally promote heterotrophic microorganisms, which are undesirable in RAS. A novel method using cork in fish feed helped fecal matter float, improving removal without affecting fish growth.

  • Focus: Enhancing microbial and chemical water quality
  • Innovations: 
    Improved biofilter design to better convert toxic ammonia.
    Analysis of micro-particles and their biological role in RAS.
    Study of how microbes in water affect the fish microbiome.
  • Goal: Maintain high water quality while minimizing harmful microbial interactions.

 

 

Off-Flavour : Identification of microbial hotspots and testing of fish feeds for off-flavour compounds. Isolation of bacteria capable of degrading these compounds was also part of the work.

  • Challenge: Earthy off-flavours (e.g., geosmin) reduce fish marketability.
  • Solutions:
    Identification of hotspots for off-flavour producing microbes.
    Testing of new fish feed prototypes to reduce off-flavour content.
    Isolation of off-flavour degrading bacteria to biologically eliminate these compounds.
  • Outcome: Improved taste and consumer acceptance of RAS-farmed fish.

 

 

Development of a high-throughput DNA chip to monitor pathogens, water quality indicators, and off-flavour genes (e.g., geosmin, 2-MIB). This chip can process 2304 data points simultaneously, offering rapid diagnostics for aquaculture facilities.

  • Purpose: Early detection of water quality issues, diseases, and off-flavour compounds.
    Technology: A PCR Fluidigm platform capable of analyzing 48 assays across 48 samples simultaneously (2304 data points).
  • Features:
    Detects pathogenic microorganisms.
    Monitors microbial water quality indicators.
    Quantifies genes responsible for off-flavours like geosmin and 2-MIB.
  • Impact: Enables rapid diagnostics and preventive management in RAS facilities. [orbit.dtu.dk]

 

 

  • Technologies:
    Tools to monitor stress levels in fish.
    Procedures to prevent spread of pathogens (viruses and bacteria).
    Molecular methods to detect parasites on gills and skin.
  • Integration: These data feed into the DNA chip for real-time health monitoring. [vmri.hu]
     

 

woman flowersAt the EAFP 2025 https://eafp2025.org/ project coordinator Louise von Gersdorff Jørgensen presented the results to stakeholders and fellow scientists. The project findings have been shared continuously in conferences and seminar. 

  • Aqua2024 in Copenhagen alongside other ITN projects (EATFISH and EASYTRAIN), showcasing joint research efforts.
  • A joint event with other EU aquaculture projects was held in Vienna, promoting interdisciplinary collaboration.

 

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